Extended life span of human endometrial stromal cells transfected with cloned origin-defective, temperature-sensitive simian virus 40
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چکیده
منابع مشابه
Extended life span of human endometrial stromal cells transfected with cloned origin-defective, temperature-sensitive simian virus 40.
Human endometrial stromal cells transfected with an origin-defective, temperature-sensitive simian virus 40 recombinant plasmid are dependent on T-antigen function for proliferation and at the permissive temperature have an extended life span in culture. Southern blot analysis indicates that the transfected gene is present in low copy number, possibly at a single integration site. Normal stroma...
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To facilitate understanding of the mechanisms underlying pulmonary diseases, including lung cancer and cystic fibrosis, we have transformed and characterized cultures of human tracheal epithelial cells. Cells were transfected by calcium phosphate precipitation with a plasmid containing a replication-defective simian virus 40 (SV40) genome. Colonies of cells with enhanced growth potential were i...
متن کاملGeneration and characterization of telomerase-transfected human lymphatic endothelial cells with an extended life span.
The study of lymphatic endothelial cells and lymphangiogenesis has, in the past, been hampered by the lack of lymphatic endothelial-specific markers. The recent discovery of several such markers has permitted the isolation of lymphatic endothelial cells (LECs) from human skin. However, cell numbers are limited and purity is variable with the different isolation procedures. To overcome these pro...
متن کاملSimian Virus 40 - Transformed Adherent Cells From Human Long - term Marrow Cultures : Cloned Cell Lines
Adherent cells from long-term marrow cultures from 23 individuals were transformed with wild-type simian virus 40 (SV4O). After transformation. cloned cell lines were developed that even after rigorous subcloning invariably produced both stromal cells and round cells. The stromal cells expressed cytoskeletal filaments similar to those of long-term marrow culture adherent cells and produced inte...
متن کاملTemperature-sensitive mutants of simian virus 40: infection of permissive cells.
Ten temperature-sensitive mutants of simian virus 40 have been isolated and characterized in permissive cells. The mutants could be divided into three functional groups and two complementation groups. Seven mutants produced T antigen, infectious viral deoxyribonucleic acid (DNA), and structural viral antigen but predominantly the empty shell type of viral particles. Two mutants produced T antig...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1991
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.65.3.1458-1465.1991